Circular Chloroplast DNA from Euglena gracilis

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Circular chloroplast DNA from Euglena gracilis.

Chloroplast DNA of the protozoan flagellate, Euglena gracilis, exists as circular molecules, 40 mum in contour length, as shown by electron microscopy and buoyant density analyses.

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Complete sequence of Euglena gracilis chloroplast DNA.

We report the complete DNA sequence of the Euglena gracilis, Pringsheim strain Z chloroplast genome. This circular DNA is 143,170 bp, counting only one copy of a 54 bp tandem repeat sequence that is present in variable copy number within a single culture. The overall organization of the genome involves a tandem array of three complete and one partial ribosomal RNA operons, and a large single co...

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Extrachromosomal circular nuclear rDNA in Euglena gracilis.

The presence of extrachromosomal nuclear ribosomal DNA (rDNA) in the unicellular alga Euglena gracilis has been established. This rDNA is circular. Each circle is 3.8 micron long and contains one rDNA unit. Oligomers are rare. Extrachromosomal rDNA is present in large amounts during the exponential phase of growth and appears less abundant during the stationary phase. It was found in all wild-t...

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Action of Nalidixic Acid on Chloroplast Replication in Euglena gracilis.

The role of light in nalidixic acid bleaching of Euglena gracilis var. bacillaris was investigated. The kinetics of loss of the chloroplast-associated DNA and the sensitivity of chloroplast replication to ultraviolet light was followed during treatment with nalidixic acid. By using the mutant P(4)ZUL, and 3-(3,4-dichlorophenyl)-, 1-dimethylurea, it was demonstrated that the requirement for ligh...

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Chloroplast.Free Preparation from Euglena gracilis'

A bstract. A supernatant fraction, free of plstids, was prepared by centrifugation from Euglena gracilis and used to ascertain whether or not the inhibition of carbon dioxide fixation by oxygen, known as the Warburg effect, is entirely independent of the light-driven phase of photosyn-thesis. This fraction exhibited in the dark the main features of the Werburg effect; namely, an inverse relatio...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1971

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.68.6.1169